Bacteriocins from lactic acid bacteria: purification strategies and applications in food and medical industries: a review

Abstract Background Bacteriocins are generally defined as ribosomally synthesized peptides, which produced by lactic acid bacteria (LAB) that affect the growth of related or unrelated microorganisms. Conventionally, extracted bacteriocins purified precipitation, where ammonium sulphate is added to precipitate out protein from solution. Main text To achieve high purity bacteriocins, a combination with chromatography used hydrophobicity and cationic properties employed. The complexity column inside can afford resolve loss during precipitation. Recently, an aqueous two-phase system (ATPS) has been widely in purification due several advantages its operational simplicity, mild process conditions versatility. It reduces operation steps processing time yet provides recovery products provide alternative ways conventional methods downstream processing. approached food medical industry. In application, nisin, Lactococcus lactis subsp. introduced preservative natural, toxicology safe effective against gram-positive bacteria. Besides, board range industries they antibiotics probiotics. Short conclusion summary, this review focuses on separation various sources using both recent ATPS techniques. Finally, recommendations for future interesting areas research need be pursued highlighted.